The sulphatase of ox liver. 2. The purification and properties of sulphatase A

The sulphatase of ox liver. II. The purification and properties of sulphatase A.

The first paper of this series (Roy, 1953) reported the presence of two distinct fractions exhibiting sulphatase activity in an aqueous extract of an acetone powder of ox liver, and gave preliminary data on the properties of the two crude enzymes, which were separated by fractional precipitation with acetone. The present paper describes the further purification and properties of fraction A, now...

The sulphatase of ox liver. 7. The intracellular distribution of sulphatases A and B.

The sulphatase of ox liver. 3. Further observations on sulphatase B and an investigation of the origin of fractions A and B.

Human liver N-acetylglucosamine-6-sulphate sulphatase. Purification and characterization.

Human N-acetylglucosamine-6-sulphate sulphatase was purified at least 50,000-fold to homogeneity in 78% yield from liver with a simple three-step four-column procedure, which consists of a concanavalin A-Sepharose/Blue A-agarose coupled step, chromatofocusing and Cu2+-chelating Sepharose chromatography. In all, four forms were isolated and partially characterized. Forms A and B, both with a pI ...

the investigation of the relationship between type a and type b personalities and quality of translation

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